Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Due to a wide range of media coverage and large scale steroid scandals fans and experts have continued to bring the games integrity into question. Major League Baseball is a game of statistics. The entirety of a player's career is based upon the consistency and credibility of the numbers and accolades acquired during the period in which they played. "Their real impact has been at the margins: There are certainly some scrubs who wouldn't be in the majors without the juice, and we have ample evidence that at the other end of the scale, drugs can take Hall of Famers and all-time greats and help them perform at historically unprecedented levels" (La-Times). When it comes to this topic generally there are two trains of thought. Many do not see the harm with this type of substance use because it makes the game more exciting and allows athletes to reach untested potentials. On the other side of the argument many fans and experts believe the game has lost its purity because of this drug use. More recently an issue has arose with high-caliber players who have tested positive for performance-enhancing drugs are not being voted for on a hall of fame ballot. This fact has brought many to question the game's integrity. No matter the statistics and achievements produced by the certain player prior to drug use, a positive test for steroids has shown to discredit the athletes integrity and career entirely.